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Brian Roman, Ph.D.
Lynn S. Florsheim MRIS Lab
Department of Radiology, MC 2026
University of Chicago

5841 S. Maryland Ave
Chicago, IL 60637
Tel: 773-702-6906
Pager: 773-438-1582
Fax: 773-834-4097
Email: broman@uchicago.edu

Dr. Roman joined the Department of Radiology and have been establishing a physiological and molecular imaging laboratory. The laboratory’s approach is to combine modern physiological and molecular biological techniques with imaging modalities. There are two main areas of research in the laboratory which are NIH supported; using magnetic resonance to detect gene expression in the heart and the development of magnetic resonance techniques for imaging pancreatic beta cell and islet function.

In order to successfully conduct this research, the laboratory requires an array of equipment which is unique to a Radiology department and provides us the ability to conduct studies ranging from the single cell to the whole animal. The laboratory has a cell culture facility consisting of a biosafety hood, cell incubator, centrifuges, and a Zeiss fluorescence microscope. The biochemistry lab is equipped with a high speed centrifuge, quantitative real-time PCR machine, UV spectrophotometers, a microplate reader, and a gel documentation and analysis system along with associated electrophoresis equipment. The animal physiology laboratory is equipped to perform microsurgery with the aid of a Zeiss surgical microscope, associated rodent ventilator and anesthesia machine. In vivo hemodyamic measurements are made in mice using a 1.4F Millar pressure/volume transducer which is inserted into the mouse ventricle and data digitized and analyzed in real time. This is not trivial as the mouse heart is only 120 mg with a heart rate of 600-700 bpm!

The primary imaging modality used is magnetic resonance, both spectroscopy and imaging. We currently utilize the 4.7T animal scanner located in the magnetic resonance imaging and spectroscopy laboratory headed by Dr. Greg Karczmar for all in vivo cardiac and pancreatic imaging. Cellular imaging studies of isolated rodent and human pancreatic islets are conducted on a 11.7T scanner located at the University of Illinois at Chicago (see image below). We are looking forward to the acquisition of a 9.4T animal scanner which will allow us conduct molecular imaging studies not possible on our current equipment.

Updated (2007) Curriculum Vitae (pdf)

 

EDUCATION

B.A. Biology, Illinois Wesleyan University, Illinois, 1985
M.S. Physiology, University of Illinois at Urbana-Champaign, Illinois, 1988
Ph.D. Physiology, University of Illinois at Urbana-Champaign, Illinois, 1992

RESEARCH INTERESTS

magnetic resonance to detect gene expression in the heart, development of magnetic resonance techniques for imaging pancreatic beta cell and islet function

 

Awards and Honors
1993-1992 Keck Scholar, W.M. Deck Center for Advanced Training and Research in computational Biology. University of Pittsburgh, Carnegie Mellon University/Pittsburgh Supercomputing Center

1995 W.M. Keck Center for Advanced Training and Research in Computational Biology Travel Fellowship. Funds for travel to the Annual Meeting of the Society of Magnetic Resonance in Medicine in Nice, France.

Refereed Publications
1. Dawson, M.J., Fletcher, E.S., Ingkanisorn, S.S., Kmiecik, J.A., and Roman, B.B., 31P and 1H Nuclear Magnetic Resonance Spectroscipic studies of contracture-inducing agents in frog skeletal muscle. Journal of Physiology, 1989; 415:133.
2. Roman, B.B., Foley, J.M., Meyer, R.A., and A.P. Koretsky, contractile and Metabolic Effects of Increased Creatine Kinase Activeity in Mouse Skeletal Muscle. American Journal of Physiology, 1996; 270: C1236-1245.
3. Roman, B.B., and Koretsky, A.P.. The brain isoform of Creatine Kinase Rescues the Contractile Defect in Mice Lacking the Muscle Isoform Despite Lack of Localization to Myofibrils. J Biol Chem. 1997; 272(28):17790-4.
4. LaBella, J.J., Daood, M.J., Koretsky, A.P., Roman, B.B., Sieck, G.C., Wieringa, B., and Watchko, J.F. Absence of Myofibrillar Creatine Kinase and Diaphragm Isometric Function During Repetitive Activation. Journal of Applied Physiology, 1998; 84(4), 116-1173.
5. Slawson, S.S., Roman, B.B., Williams, D.S., and Koretsky, A.P. Cardiac MRI of the Normal and Hypertrophied Mouse Heart. Magnetic Resonance in Medicine, 1998; 39; 980-987.
6. Roman, B.B., Geenen, D.L., Leitges, M., and Buttrick, PlM. PKC-B is not necessary for Cardiac Hypertrophy. American Journal of Physiology – Heart and Circ, 2001; 280: H2264-H2270.
7. Montgomery, D.E., Wolska, B.M., Pyle, W.G., Roman, B.B., Dowell, J.C., Buttrick, PlM., Koretsky, A.P., Del Nido, P., and Solaro, R.J. Alpha-Adrenergic Response and Myofilament Activity in Mouse Hearts Lacking PKC Phosphorylation Sites on Cardiac TnI. Am J Physiol Heart Circ Physiol, 2002; 282: H2397-405.
8. Roman, B.B., Meyer, R.A., and Wiseman, R. Phosphocreatine Kinetics at the Onset of Contraction in Skeletal Muscle of MM Creatine Kinase Knockout Mice. Am J Physiol Cell Physiol, 2002; 283(6); C1776-83.
9. Eroglu, S., Gimi, B., Roman, B.B., Friedman, G., and Magin, R. NMR Spiral Microcoils: Design, Fabrication, and Imaging. Concepts in Magnetic Resonance, 2003; 17B:1-10.
10. Gimi, B., Eroglu, S., Leoni, L., Desai, T.A., Magin, R.L., and Roman, B.B. NMR Sprial Surface Microcoils: Applications. Concepts in Magnetic Resonance, 2003; 18B: 1-18.
11. Itani S.I., Tapscott E.B., Leitges M., McKinney R., Roman B.B., Buttrick, P.M., and Dohm G.L. Effect of Protein Kinase C Beta Knckout on Insulin Signal Transduction in Transgnic Mice. Amer J Physiol, 2003; In revision.
12. Roman B.B., Goldspink, P.H., Spaite, E., Urboniene, D., McKinney, R., Geenen, D.L., Solaro R.J., and Buttrick P.M. Inhibition of PKC Phosphorylation of cTnI Improves Cardiac Performance In Vivo. American Journal of Physiology: Heart, 2004 286: H2089-H2095.
13. Barjor G., Leoni L., Oberholzer J., Braun M., Avila J., Wang Y., Desai T., Philipson L.H., Magin R.L., and Roman B.B. Functional MR Microimaging of Pancreatic B-Cell Activation. Cell Transplantation the Regenerative Medicine Journal, 2006; 15: 195-203.
14. Fan, X., Markiewicz, E., Haque, M., Zamora, M., Karczmar, G.S., Roman, B.B. Open Birdcage Coil and Physiological Chamber for Mouse Cardiac Imaging. Journal of Cardiovascular Magnetic Resonance, 2006; 8(1); 298-299.
15. Fan X., Markiewicz E.J., Zamora M., Karczmar G., Roman B.B. Comparison and Evaluation of Mouse Cardiac MRI Acquired With Open Birdcage, Single Loop Surface And Volume Birdcage Coils, 2006; 51; N451-N459.
16. LaFlamme K., Leoni L., Popat K., Markiewicz E.J., Roman B.B., Desai T.A. Biocompatibility of Porous Alumina Biocapsules, 2006 (In Press).

Grant Support
Current

2003-2008 Imaging Pancreatic B-Cell Function by Magnetic Resonance
NIH 1 R01 EB0011828-01: $1,250, 000
Role: PI

2003-2008 NMR Detection of Gene Expressions
NIH 1 R01 HL0609961-01: $1,328,334
Role: PI

2005-2006 Quantitative and Fast MRI Imaging of Pancreas Cancer
Cancer Research Center Pilot Grant (PI: Pan)
University of Chicago $30,000
Role: Co-Investigator

Completed

1994-1997 National Institutes of Health Postdoctoral Fellowship (NRSA)

1999-2001 Protein Kinase C Effects on Senescent Heart Function
NIH 1R03AG016443-01 $100,000
Role: Co-Investigator

2002-2004 Magnetic Resonance Imaging of Beta-Cell Activation
ADA Innovation Award $100,000
Role: PI